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Cryo-EM sample preparation and data collection Sucrose gradient purified fractions of the PV3-SC8 or wt PV2 VLPs expressed in yeast and mammalian cells, respectively, were pooled, buffer exchanged into DPBS-EDTA using Zeba Spin Desalting Columns (Thermo Fisher Scientific) with a 7 K molecular weight cut-off (MWCO) and concentrated using Amicon Ultra centrifugal filter devices (100 kDa MWCO, Merck Millipore) to a concentration of 0.91.5 mg/ml

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However, it is important to emphasize that, at present, robust clinical evidence for dual and triple agonists is derived predominantly from adult populations

A pre-existing ClaI restriction site in the TS sequence was utilised to fuse the first, N-terminal, Rex domain of NAPstar4 to the second, C-terminal, Rex domain of NAPstar3 to generate the NAPstar4.3 construct

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These broader systemic effects of semaglutide may inadvertently create a biological environment that is more conducive to the growth of yeast, particularly Candida species