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Compound 22 , a hybrid with the N-terminal from 21 and the C-terminal from 16 demonstrated a further enhanced potency (Ki = 23 nM) and a high selectivity for IRAP over AP-N ( 16 , devoid of a macrocyclic ring system in the N-terminal is not selective and inhibits both IRAP and AP-N activity, suggesting that introduction of proper conformational constrains in the N-terminal by macrocyclization improves selectivity ( 15 and Ang IV seem to adopt a -turn at the C-terminal when binding to IRAP, while in the case of 21 , a less well-defined turn conformation is adopted at the N-terminal

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Its B ring displays the catechol portion and multiple free hydroxyl groups that drive the reduction of Fe 3+ to Fe 2+ (73)