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After centrifugation at 15,700 g for 5 min the supernatant was discarded and the cell pellet was repeatedly washed in 5 mL of TE buffer and resuspended in 3 mL of lithium/caesium acetate solution (Qbiogene)

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Diabetes Mellitus (DM) DM is a metabolic disease characterized by long-term hyperglycemia, leading to serious complications such as microangiopathy and macroangiopathy, significantly increasing mortality [141]