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Mouse islets were transduced 24 hours prior to imaging with an adenoviral SNAP/FLAG-hGLP-1R vector (custom made by VectorBuilder) at a MOI of 1 and subsequently labelled for 10 minutes at 37 C with SNAP-Surface Alexa Fluor 647 before 5-minute stimulation with indicated agonists and imaging of whole islets in the same microscope as above

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Specifically, IFN- activates macrophages, neutrophils, and dendritic cells (DC) to destroy intracellular pathogens by producing reactive oxygen species (ROS), reactive nitrogen species, and antimicrobial peptides

Furthermore, it prevented apoptosis and reduced the expression of microRNA (miR-339-5p) that is involved in programmed cell death

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