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In contrast, when utilising 30% U- 13 CR 6 -labelled arginine as the sole carbon source, there were only a few differences between the metabolic labelling in gdh3 / gdh3 and wild-type cells that included two TCA cycle intermediates (fumarate and cis -aconitate) and all three aromatic amino acids (phenylalanine, tryptophan, and tyrosine)

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The dynamic range of the human metabolome revealed by challenges

On the other hand, the wild-type strain could relieve the high NAD + usage by employing Gdh3 which is dependent on NADP +

Gene 67, 3140

10.3892/etm.2021.10503 144 XiaY.YangW.BuW.JiH.ZhaoX.ZhengY.et al (2013)