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Lastly, all three purified DNA fragments were joined together by fusion PCR 23 using primers P1 and P4 to complete the construction of SAT1 flipper disruption cassette

But it changes the entire workflow

[DOI] [PMC free article] [PubMed] [Google Scholar] 636.Pop-Busui, R

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C-met binding was examined at two HGF-doses (250 ng/ml and 500 ng/ml in PBS) by sandwich ELISA-like assay on recombinant c-met-Fc chimera as in Figure 1e, and significant changes in optical absorbance subtracted by a baseline level (negative control without HGF, bar 1 ) were depicted in Figure 2a (left column)