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thousands in primary care), allows use of surrogate endpoints with accelerated approval, and creates a more predictable regulatory dialogue

The PVDF membranes were blocked with 5% nonfat milk for 1.5 h and then incubated overnight at 4 C with the following primary antibodies: anti-TNF- (Abcam, ab300093), anti-NLRP3 (Abcam, ab254360), anti-BDNF (Abcam, ab108319), anti-Synaptophysin (SYP) (Abcam, ab156302) and anti-PSD95 (Abcam, ab238135)