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To account for signal variation across experiments and due to the use of two different cameras along this experimental series, values were normalized within each independent experiment by dividing each individual measurement by the mean nuclear YAP intensity across all conditions

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To prepare for rigidity and allostery analysis, missing hydrogen atoms were added to Cryo-EM structure using WHAT IF web server ( We sequentially perturbed the rigidity of a window of the three-consecutive residue starting from N-terminus, to calculate maximum degrees of freedom that can be transmitted to the GTP binding region

Ann Thorac Surg 89(6):1797-1803, 1804.e1-3

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Simultaneously, DAG is believed to activate protein kinase C (PKC), a family of enzymes implicated in various cellular functions