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Beta-carotene serum levels in patients with erythropoietic protoporphyria on treatment with the synthetic all-trans isomer or a natural isomeric mixture of beta-carotene

The expression of TLR2 was detected by flow cytometry, and the positive expression level was counted

A 20 l aliquot of trypsinized cells was transferred to a microcentrifuge tube containing an equal amount of 0.4% trypan blue dye, mixed and loaded onto a hemocytometer